Review



vectashield plus dapi mounting media  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 98
      Buy from Supplier

    Structured Review

    Vector Laboratories vectashield plus dapi mounting media
    Cell cycle dependencies upon CAF-1 and SPT6 perturbations. ( A ) iGMPs were arrested with 0.1× or 0.01× SCF (arrest), in parallel with normal 1× SCF treatment. For SPT6 KD and CAF-1 KD conditions, arrest was performed following 12 h of pretreatment with Dox and IPTG, respectively. For neutrophil differentiation conditions, cells were arrested for 12 h before inducing differentiation for 36 h. SCF concentrations were maintained throughout the 48 h (arrest). Samples were collected “postarrest” to confirm arrest (see ) and at the “end point” as shown in the schematic. ( B ) Histograms showing cKit and GFP expression quantified by flow cytometry at the “end point” of each treatment, as shown in A . iGMP, SPT6 KD, CAF-1 KD, and neutrophil histograms are overlaid for each SCF condition. ( C ) Quantification of GFP and cKit expression levels shown in B for each treatment relative to 1× SCF. n = 2 clonal replicates. ( D ) Flow cytometry contour plot showing incorporation of 5-ethynyluridine (EU) versus total DNA <t>DAPI</t> staining in iGMPs and after 48 h of SPT6 KD, CAF-1 KD, and neutrophil differentiation. Untreated iGMPs are overlaid with each condition and colored accordingly. ( E ) Time-course analysis of EU incorporation relative to iGMPs for each condition as a function of cell cycle phase. n = 2 clonal replicates. Phases were gated based on DAPI signal (see also histograms in ). ( F ) Cell cycle plots showing EdU incorporation and total DNA staining (DAPI) quantified with flow cytometry. G1/G0, S, and G2/M cell cycle phases are gated based on EdU and DAPI signal. Data were quantified in iGMPs and after 24 or 48 h of continuous treatment for each condition. Representative of n = 2 clonal replicates. The experiment was independently repeated three times with consistent results. (AF647) Alexa fluor-647. ( G ) The percentage of cells in each cell cycle phase as gated in F . The mean of n = 2 clonal replicates with error bars representing the range. ( H ) Histograms quantifying DNA content in the bulk population for each condition and EdU incorporation in S-phase cells from cells shown in F . Representative of n = 2 clonal replicates. ( I ) Geometric mean fluorescence intensity of EdU incorporation in S-phase cells as shown in H for n = 2 clonal replicates. ( J ) Cell cycle plots as shown in F , but with GFP − (gray) and GFP + (green) cells displayed for each condition and time point. A reduced number of events is shown for clarity (see quantification of total populations in ).
    Vectashield Plus Dapi Mounting Media, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 21065 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield plus dapi mounting media/product/Vector Laboratories
    Average 98 stars, based on 21065 article reviews
    vectashield plus dapi mounting media - by Bioz Stars, 2026-03
    98/100 stars

    Images

    1) Product Images from "Histone chaperones coupled to DNA replication and transcription control divergent chromatin elements to maintain cell fate"

    Article Title: Histone chaperones coupled to DNA replication and transcription control divergent chromatin elements to maintain cell fate

    Journal: Genes & Development

    doi: 10.1101/gad.352316.124

    Cell cycle dependencies upon CAF-1 and SPT6 perturbations. ( A ) iGMPs were arrested with 0.1× or 0.01× SCF (arrest), in parallel with normal 1× SCF treatment. For SPT6 KD and CAF-1 KD conditions, arrest was performed following 12 h of pretreatment with Dox and IPTG, respectively. For neutrophil differentiation conditions, cells were arrested for 12 h before inducing differentiation for 36 h. SCF concentrations were maintained throughout the 48 h (arrest). Samples were collected “postarrest” to confirm arrest (see ) and at the “end point” as shown in the schematic. ( B ) Histograms showing cKit and GFP expression quantified by flow cytometry at the “end point” of each treatment, as shown in A . iGMP, SPT6 KD, CAF-1 KD, and neutrophil histograms are overlaid for each SCF condition. ( C ) Quantification of GFP and cKit expression levels shown in B for each treatment relative to 1× SCF. n = 2 clonal replicates. ( D ) Flow cytometry contour plot showing incorporation of 5-ethynyluridine (EU) versus total DNA DAPI staining in iGMPs and after 48 h of SPT6 KD, CAF-1 KD, and neutrophil differentiation. Untreated iGMPs are overlaid with each condition and colored accordingly. ( E ) Time-course analysis of EU incorporation relative to iGMPs for each condition as a function of cell cycle phase. n = 2 clonal replicates. Phases were gated based on DAPI signal (see also histograms in ). ( F ) Cell cycle plots showing EdU incorporation and total DNA staining (DAPI) quantified with flow cytometry. G1/G0, S, and G2/M cell cycle phases are gated based on EdU and DAPI signal. Data were quantified in iGMPs and after 24 or 48 h of continuous treatment for each condition. Representative of n = 2 clonal replicates. The experiment was independently repeated three times with consistent results. (AF647) Alexa fluor-647. ( G ) The percentage of cells in each cell cycle phase as gated in F . The mean of n = 2 clonal replicates with error bars representing the range. ( H ) Histograms quantifying DNA content in the bulk population for each condition and EdU incorporation in S-phase cells from cells shown in F . Representative of n = 2 clonal replicates. ( I ) Geometric mean fluorescence intensity of EdU incorporation in S-phase cells as shown in H for n = 2 clonal replicates. ( J ) Cell cycle plots as shown in F , but with GFP − (gray) and GFP + (green) cells displayed for each condition and time point. A reduced number of events is shown for clarity (see quantification of total populations in ).
    Figure Legend Snippet: Cell cycle dependencies upon CAF-1 and SPT6 perturbations. ( A ) iGMPs were arrested with 0.1× or 0.01× SCF (arrest), in parallel with normal 1× SCF treatment. For SPT6 KD and CAF-1 KD conditions, arrest was performed following 12 h of pretreatment with Dox and IPTG, respectively. For neutrophil differentiation conditions, cells were arrested for 12 h before inducing differentiation for 36 h. SCF concentrations were maintained throughout the 48 h (arrest). Samples were collected “postarrest” to confirm arrest (see ) and at the “end point” as shown in the schematic. ( B ) Histograms showing cKit and GFP expression quantified by flow cytometry at the “end point” of each treatment, as shown in A . iGMP, SPT6 KD, CAF-1 KD, and neutrophil histograms are overlaid for each SCF condition. ( C ) Quantification of GFP and cKit expression levels shown in B for each treatment relative to 1× SCF. n = 2 clonal replicates. ( D ) Flow cytometry contour plot showing incorporation of 5-ethynyluridine (EU) versus total DNA DAPI staining in iGMPs and after 48 h of SPT6 KD, CAF-1 KD, and neutrophil differentiation. Untreated iGMPs are overlaid with each condition and colored accordingly. ( E ) Time-course analysis of EU incorporation relative to iGMPs for each condition as a function of cell cycle phase. n = 2 clonal replicates. Phases were gated based on DAPI signal (see also histograms in ). ( F ) Cell cycle plots showing EdU incorporation and total DNA staining (DAPI) quantified with flow cytometry. G1/G0, S, and G2/M cell cycle phases are gated based on EdU and DAPI signal. Data were quantified in iGMPs and after 24 or 48 h of continuous treatment for each condition. Representative of n = 2 clonal replicates. The experiment was independently repeated three times with consistent results. (AF647) Alexa fluor-647. ( G ) The percentage of cells in each cell cycle phase as gated in F . The mean of n = 2 clonal replicates with error bars representing the range. ( H ) Histograms quantifying DNA content in the bulk population for each condition and EdU incorporation in S-phase cells from cells shown in F . Representative of n = 2 clonal replicates. ( I ) Geometric mean fluorescence intensity of EdU incorporation in S-phase cells as shown in H for n = 2 clonal replicates. ( J ) Cell cycle plots as shown in F , but with GFP − (gray) and GFP + (green) cells displayed for each condition and time point. A reduced number of events is shown for clarity (see quantification of total populations in ).

    Techniques Used: Expressing, Flow Cytometry, Staining, Fluorescence



    Similar Products

    vectashield plus dapi mounting media  (Vector Laboratories)
    98
    Vector Laboratories vectashield plus dapi mounting media
    Cell cycle dependencies upon CAF-1 and SPT6 perturbations. ( A ) iGMPs were arrested with 0.1× or 0.01× SCF (arrest), in parallel with normal 1× SCF treatment. For SPT6 KD and CAF-1 KD conditions, arrest was performed following 12 h of pretreatment with Dox and IPTG, respectively. For neutrophil differentiation conditions, cells were arrested for 12 h before inducing differentiation for 36 h. SCF concentrations were maintained throughout the 48 h (arrest). Samples were collected “postarrest” to confirm arrest (see ) and at the “end point” as shown in the schematic. ( B ) Histograms showing cKit and GFP expression quantified by flow cytometry at the “end point” of each treatment, as shown in A . iGMP, SPT6 KD, CAF-1 KD, and neutrophil histograms are overlaid for each SCF condition. ( C ) Quantification of GFP and cKit expression levels shown in B for each treatment relative to 1× SCF. n = 2 clonal replicates. ( D ) Flow cytometry contour plot showing incorporation of 5-ethynyluridine (EU) versus total DNA <t>DAPI</t> staining in iGMPs and after 48 h of SPT6 KD, CAF-1 KD, and neutrophil differentiation. Untreated iGMPs are overlaid with each condition and colored accordingly. ( E ) Time-course analysis of EU incorporation relative to iGMPs for each condition as a function of cell cycle phase. n = 2 clonal replicates. Phases were gated based on DAPI signal (see also histograms in ). ( F ) Cell cycle plots showing EdU incorporation and total DNA staining (DAPI) quantified with flow cytometry. G1/G0, S, and G2/M cell cycle phases are gated based on EdU and DAPI signal. Data were quantified in iGMPs and after 24 or 48 h of continuous treatment for each condition. Representative of n = 2 clonal replicates. The experiment was independently repeated three times with consistent results. (AF647) Alexa fluor-647. ( G ) The percentage of cells in each cell cycle phase as gated in F . The mean of n = 2 clonal replicates with error bars representing the range. ( H ) Histograms quantifying DNA content in the bulk population for each condition and EdU incorporation in S-phase cells from cells shown in F . Representative of n = 2 clonal replicates. ( I ) Geometric mean fluorescence intensity of EdU incorporation in S-phase cells as shown in H for n = 2 clonal replicates. ( J ) Cell cycle plots as shown in F , but with GFP − (gray) and GFP + (green) cells displayed for each condition and time point. A reduced number of events is shown for clarity (see quantification of total populations in ).
    Vectashield Plus Dapi Mounting Media, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield plus dapi mounting media/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    vectashield plus dapi mounting media - by Bioz Stars, 2026-03
    98/100 stars
    		  Buy from Supplier
    vectashield plus antifade mounting media with dapi  (Vector Laboratories)
    98
    Vector Laboratories vectashield plus antifade mounting media with dapi
    Cell cycle dependencies upon CAF-1 and SPT6 perturbations. ( A ) iGMPs were arrested with 0.1× or 0.01× SCF (arrest), in parallel with normal 1× SCF treatment. For SPT6 KD and CAF-1 KD conditions, arrest was performed following 12 h of pretreatment with Dox and IPTG, respectively. For neutrophil differentiation conditions, cells were arrested for 12 h before inducing differentiation for 36 h. SCF concentrations were maintained throughout the 48 h (arrest). Samples were collected “postarrest” to confirm arrest (see ) and at the “end point” as shown in the schematic. ( B ) Histograms showing cKit and GFP expression quantified by flow cytometry at the “end point” of each treatment, as shown in A . iGMP, SPT6 KD, CAF-1 KD, and neutrophil histograms are overlaid for each SCF condition. ( C ) Quantification of GFP and cKit expression levels shown in B for each treatment relative to 1× SCF. n = 2 clonal replicates. ( D ) Flow cytometry contour plot showing incorporation of 5-ethynyluridine (EU) versus total DNA <t>DAPI</t> staining in iGMPs and after 48 h of SPT6 KD, CAF-1 KD, and neutrophil differentiation. Untreated iGMPs are overlaid with each condition and colored accordingly. ( E ) Time-course analysis of EU incorporation relative to iGMPs for each condition as a function of cell cycle phase. n = 2 clonal replicates. Phases were gated based on DAPI signal (see also histograms in ). ( F ) Cell cycle plots showing EdU incorporation and total DNA staining (DAPI) quantified with flow cytometry. G1/G0, S, and G2/M cell cycle phases are gated based on EdU and DAPI signal. Data were quantified in iGMPs and after 24 or 48 h of continuous treatment for each condition. Representative of n = 2 clonal replicates. The experiment was independently repeated three times with consistent results. (AF647) Alexa fluor-647. ( G ) The percentage of cells in each cell cycle phase as gated in F . The mean of n = 2 clonal replicates with error bars representing the range. ( H ) Histograms quantifying DNA content in the bulk population for each condition and EdU incorporation in S-phase cells from cells shown in F . Representative of n = 2 clonal replicates. ( I ) Geometric mean fluorescence intensity of EdU incorporation in S-phase cells as shown in H for n = 2 clonal replicates. ( J ) Cell cycle plots as shown in F , but with GFP − (gray) and GFP + (green) cells displayed for each condition and time point. A reduced number of events is shown for clarity (see quantification of total populations in ).
    Vectashield Plus Antifade Mounting Media With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield plus antifade mounting media with dapi/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    vectashield plus antifade mounting media with dapi - by Bioz Stars, 2026-03
    98/100 stars
    		  Buy from Supplier
    vectashield plus antifade mounting media  (Vector Laboratories)
    98
    Vector Laboratories vectashield plus antifade mounting media
    Cell cycle dependencies upon CAF-1 and SPT6 perturbations. ( A ) iGMPs were arrested with 0.1× or 0.01× SCF (arrest), in parallel with normal 1× SCF treatment. For SPT6 KD and CAF-1 KD conditions, arrest was performed following 12 h of pretreatment with Dox and IPTG, respectively. For neutrophil differentiation conditions, cells were arrested for 12 h before inducing differentiation for 36 h. SCF concentrations were maintained throughout the 48 h (arrest). Samples were collected “postarrest” to confirm arrest (see ) and at the “end point” as shown in the schematic. ( B ) Histograms showing cKit and GFP expression quantified by flow cytometry at the “end point” of each treatment, as shown in A . iGMP, SPT6 KD, CAF-1 KD, and neutrophil histograms are overlaid for each SCF condition. ( C ) Quantification of GFP and cKit expression levels shown in B for each treatment relative to 1× SCF. n = 2 clonal replicates. ( D ) Flow cytometry contour plot showing incorporation of 5-ethynyluridine (EU) versus total DNA <t>DAPI</t> staining in iGMPs and after 48 h of SPT6 KD, CAF-1 KD, and neutrophil differentiation. Untreated iGMPs are overlaid with each condition and colored accordingly. ( E ) Time-course analysis of EU incorporation relative to iGMPs for each condition as a function of cell cycle phase. n = 2 clonal replicates. Phases were gated based on DAPI signal (see also histograms in ). ( F ) Cell cycle plots showing EdU incorporation and total DNA staining (DAPI) quantified with flow cytometry. G1/G0, S, and G2/M cell cycle phases are gated based on EdU and DAPI signal. Data were quantified in iGMPs and after 24 or 48 h of continuous treatment for each condition. Representative of n = 2 clonal replicates. The experiment was independently repeated three times with consistent results. (AF647) Alexa fluor-647. ( G ) The percentage of cells in each cell cycle phase as gated in F . The mean of n = 2 clonal replicates with error bars representing the range. ( H ) Histograms quantifying DNA content in the bulk population for each condition and EdU incorporation in S-phase cells from cells shown in F . Representative of n = 2 clonal replicates. ( I ) Geometric mean fluorescence intensity of EdU incorporation in S-phase cells as shown in H for n = 2 clonal replicates. ( J ) Cell cycle plots as shown in F , but with GFP − (gray) and GFP + (green) cells displayed for each condition and time point. A reduced number of events is shown for clarity (see quantification of total populations in ).
    Vectashield Plus Antifade Mounting Media, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vectashield plus antifade mounting media/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    vectashield plus antifade mounting media - by Bioz Stars, 2026-03
    98/100 stars
    		  Buy from Supplier

    Image Search Results


    Cell cycle dependencies upon CAF-1 and SPT6 perturbations. ( A ) iGMPs were arrested with 0.1× or 0.01× SCF (arrest), in parallel with normal 1× SCF treatment. For SPT6 KD and CAF-1 KD conditions, arrest was performed following 12 h of pretreatment with Dox and IPTG, respectively. For neutrophil differentiation conditions, cells were arrested for 12 h before inducing differentiation for 36 h. SCF concentrations were maintained throughout the 48 h (arrest). Samples were collected “postarrest” to confirm arrest (see ) and at the “end point” as shown in the schematic. ( B ) Histograms showing cKit and GFP expression quantified by flow cytometry at the “end point” of each treatment, as shown in A . iGMP, SPT6 KD, CAF-1 KD, and neutrophil histograms are overlaid for each SCF condition. ( C ) Quantification of GFP and cKit expression levels shown in B for each treatment relative to 1× SCF. n = 2 clonal replicates. ( D ) Flow cytometry contour plot showing incorporation of 5-ethynyluridine (EU) versus total DNA DAPI staining in iGMPs and after 48 h of SPT6 KD, CAF-1 KD, and neutrophil differentiation. Untreated iGMPs are overlaid with each condition and colored accordingly. ( E ) Time-course analysis of EU incorporation relative to iGMPs for each condition as a function of cell cycle phase. n = 2 clonal replicates. Phases were gated based on DAPI signal (see also histograms in ). ( F ) Cell cycle plots showing EdU incorporation and total DNA staining (DAPI) quantified with flow cytometry. G1/G0, S, and G2/M cell cycle phases are gated based on EdU and DAPI signal. Data were quantified in iGMPs and after 24 or 48 h of continuous treatment for each condition. Representative of n = 2 clonal replicates. The experiment was independently repeated three times with consistent results. (AF647) Alexa fluor-647. ( G ) The percentage of cells in each cell cycle phase as gated in F . The mean of n = 2 clonal replicates with error bars representing the range. ( H ) Histograms quantifying DNA content in the bulk population for each condition and EdU incorporation in S-phase cells from cells shown in F . Representative of n = 2 clonal replicates. ( I ) Geometric mean fluorescence intensity of EdU incorporation in S-phase cells as shown in H for n = 2 clonal replicates. ( J ) Cell cycle plots as shown in F , but with GFP − (gray) and GFP + (green) cells displayed for each condition and time point. A reduced number of events is shown for clarity (see quantification of total populations in ).

    Journal: Genes & Development

    Article Title: Histone chaperones coupled to DNA replication and transcription control divergent chromatin elements to maintain cell fate

    doi: 10.1101/gad.352316.124

    Figure Lengend Snippet: Cell cycle dependencies upon CAF-1 and SPT6 perturbations. ( A ) iGMPs were arrested with 0.1× or 0.01× SCF (arrest), in parallel with normal 1× SCF treatment. For SPT6 KD and CAF-1 KD conditions, arrest was performed following 12 h of pretreatment with Dox and IPTG, respectively. For neutrophil differentiation conditions, cells were arrested for 12 h before inducing differentiation for 36 h. SCF concentrations were maintained throughout the 48 h (arrest). Samples were collected “postarrest” to confirm arrest (see ) and at the “end point” as shown in the schematic. ( B ) Histograms showing cKit and GFP expression quantified by flow cytometry at the “end point” of each treatment, as shown in A . iGMP, SPT6 KD, CAF-1 KD, and neutrophil histograms are overlaid for each SCF condition. ( C ) Quantification of GFP and cKit expression levels shown in B for each treatment relative to 1× SCF. n = 2 clonal replicates. ( D ) Flow cytometry contour plot showing incorporation of 5-ethynyluridine (EU) versus total DNA DAPI staining in iGMPs and after 48 h of SPT6 KD, CAF-1 KD, and neutrophil differentiation. Untreated iGMPs are overlaid with each condition and colored accordingly. ( E ) Time-course analysis of EU incorporation relative to iGMPs for each condition as a function of cell cycle phase. n = 2 clonal replicates. Phases were gated based on DAPI signal (see also histograms in ). ( F ) Cell cycle plots showing EdU incorporation and total DNA staining (DAPI) quantified with flow cytometry. G1/G0, S, and G2/M cell cycle phases are gated based on EdU and DAPI signal. Data were quantified in iGMPs and after 24 or 48 h of continuous treatment for each condition. Representative of n = 2 clonal replicates. The experiment was independently repeated three times with consistent results. (AF647) Alexa fluor-647. ( G ) The percentage of cells in each cell cycle phase as gated in F . The mean of n = 2 clonal replicates with error bars representing the range. ( H ) Histograms quantifying DNA content in the bulk population for each condition and EdU incorporation in S-phase cells from cells shown in F . Representative of n = 2 clonal replicates. ( I ) Geometric mean fluorescence intensity of EdU incorporation in S-phase cells as shown in H for n = 2 clonal replicates. ( J ) Cell cycle plots as shown in F , but with GFP − (gray) and GFP + (green) cells displayed for each condition and time point. A reduced number of events is shown for clarity (see quantification of total populations in ).

    Article Snippet: Coverslips were mounted with VectaShield Plus DAPI mounting media (Vector Laboratories H-2000), and images were acquired on a Leica DMi8 microscope with Leica application suite X (v3.3).

    Techniques: Expressing, Flow Cytometry, Staining, Fluorescence